Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer

Ki-Hyuk Shin , Reuben H. Kim , Bo Yu , Mo K. Kang , David Elashoff , Russell Christensen , No-Hee Park , Ana Pucar DDS, MSc, PhD

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We previously reported that wild type (wt) hnRNP G exhibited tumor suppressive activity in human oral squamous cell carcinoma (HOSCC) cell lines lacking hnRNP G. Wt hnRNP G markedly inhibited the proliferation capacity, anchorage independency and in vivo tumorigenicity of HOSCC cells and notably enhanced the DNA repair capabilities of these cells. In the present study, we studied the genetic and expression states of hnRNP G in normal, premalignant and malignant human oral tissues to further understand the relationship between the hnRNP G alterations and the development of human oral cancer. To correlate the cancer development and the level of hnRNP G expression, we performed an immunohistochemistry staining of hnRNP G in normal, premalignant and malignant human oral tissues. Moreover, we examined the entire coding regions of hnRNP G from selected samples to understand the cause of the alterations of the gene expression.

The expression of hnRNP G was notably decreased or completely abolished in 80% of premalignant-dysplastic and malignant oral epithelial tissues, whereas 100% of normal and 90% of hyperplastic non-dysplastic epithelium showed high level of hnRNP G in the nucleus of the basal cell layers. Approximately 80% of HOSCC lacking the expression of hnRNP G showed genetic alteration in hnRNP G, i.e., point mutation and exonic deletion. This study suggest that genetic alterations and aberrant expression of hnRNP G occurring during oral carcinogenesis might be useful markers for the early detection of human oral cancer.

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Fecha de comienzo: 07 mayo 2015
Fecha de expiración: 06 mayo 2018

Author(s)

Ki-Hyuk Shin

  • School of Dentistry, University of California, Los Angeles, CA, USA
  • Dental Research Institute, University of California, Los Angeles, CA, USA
  • Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA

Reuben H. Kim

  • School of Dentistry, University of California, Los Angeles, CA, USA
  • Dental Research Institute, University of California, Los Angeles, CA, USA
  • Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA

Bo Yu

  • School of Dentistry, University of California, Los Angeles, CA, USA

Mo K. Kang

  • School of Dentistry, University of California, Los Angeles, CA, USA
  • Dental Research Institute, University of California, Los Angeles, CA, USA
  • Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA

David Elashoff

  • David Geffen School of Medicine, University of California, Los Angeles, CA, USA

Russell Christensen

  • School of Dentistry, University of California, Los Angeles, CA, USA

No-Hee Park

  • School of Dentistry, University of California, Los Angeles, CA, USA
  • Dental Research Institute, University of California, Los Angeles, CA, USA
  • Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA
  • David Geffen School of Medicine, University of California, Los Angeles, CA, USA

Ana Pucar DDS, MSc, PhD

1996. Dr. Ana Pucar graduated in School of Dental Medicine, University of Belgrade, Serbia. 2000. MSc in Periodontology and Oral Medicine, School of  Dentistry, University of Belgrade on the subject of Oral manifestation of the colorectal carcinoma chemotherapy. 2001. Clinical Specialization in Periodontology and Oral Medicine. 2000. PhD in Investigation of the presence of oral microflora microorganisms as risk factor for development of occlusive arterial disease. 2008.-2009. Visiting researcher at the Dept. of Oral Biology UCLA School of Dentistry. Since 2007.  has been a part of ITOP program both nationally and internationally. Received several prizes for a research papers presented at meetings. 2010. Dr. Pucar became an Associated Professor of Oral Medicine at the Dept. of Periodontology and Oral Medicine, School of Dental Medicine, University of Belgrade Since 2011. Dr. Pucar has been working as a teacher in the Dept. of. Dentistry, Medical Faculty of Banja Luka, Bosnia and Herzegovina.  Published two student’s textbooks and several scientific papers. She is a member of European Association of Oral Medicine, Balkan Stomatological Society, European Federation of Periodontology, and Serbian Dental Association.

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